https://eln.excellgen.com/index.php?action=history&feed=atom&title=2150-pEC-Flpo-MS2Pac 2150-pEC-Flpo-MS2Pac - Revision history 2026-10-18T23:05:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2150-pEC-Flpo-MS2Pac&diff=11615&oldid=prev Paul.xu: Created page with "== '''Cloning of 2150-pEC-Flpo-MS2Pac''' == '''Purpose:''' Cloning of 2150-pEC-Flpo-MS2Pac '''Date: '''2014-4-2---2014-4-4 '''Notebook:0''' '''Page:''' '''Clone ID:2150..." 2014-04-04T18:22:51Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2150-pEC-Flpo-MS2Pac&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2150-pEC-Flpo-MS2Pac &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-2---2014-4-4 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2150...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2150-pEC-Flpo-MS2Pac&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2150-pEC-Flpo-MS2Pac <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-2---2014-4-4 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2150&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2148pcr 2149 2150 2151 4-2-2014.JPG]] <br /> <br /> PCR using 1520 pDIRE as template&lt;br&gt; <br /> <br /> MS2Pac-NLS-HindIII-F &lt;br&gt; <br /> <br /> Flpo-MS2Pac-Xho1-R&lt;br&gt; <br /> <br /> [[Image:2149 2150 2151clone 4-2-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 3&lt;br&gt;<br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | pEC&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | HindIII + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-4 <br /> <br /> Make miniprep and digest with HindIII + Xho1<br /> <br /> [[Image:2149 2150 2128digest 4-4-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu