https://eln.excellgen.com/index.php?action=history&feed=atom&title=2155-pEC2-6H-tat-sc-yMS2 2155-pEC2-6H-tat-sc-yMS2 - Revision history 2026-10-18T14:44:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2155-pEC2-6H-tat-sc-yMS2&diff=11658&oldid=prev Paul.xu: Created page with "== '''Cloning of 2155-pEC2-6H-tat-sc-yMS2''' == '''Purpose:''' Cloning of 2155-pEC2-6H-tat-sc-yMS2 '''Date: '''2014-4-4---2014-4-9 '''Notebook:0''' '''Page:''' '''Clone I..." 2014-04-09T19:50:31Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2155-pEC2-6H-tat-sc-yMS2&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2155-pEC2-6H-tat-sc-yMS2 &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-4---2014-4-9 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone I...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2155-pEC2-6H-tat-sc-yMS2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2155-pEC2-6H-tat-sc-yMS2<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-4---2014-4-9<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2155&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2155_2157_2159PCR_4-4-2014.JPG]]<br /> <br /> &amp;nbsp;pcr1:gene synthesis yMS2-1 to&amp;nbsp; yMS2-12 &lt;br&gt;<br /> <br /> pcr2: gene synthesis GLink-MS2-F to&amp;nbsp; YMS2-EcoR1-R&lt;br&gt;<br /> <br /> [[Image:732_2145_2147_2152_2155_2174_2180PCR_4-7-2014.JPG]]&lt;br&gt;<br /> <br /> pcr1+pcr2&lt;br&gt;yMS2-1 &lt;br&gt;YMS2-EcoR1-R&lt;br&gt;<br /> <br /> [[Image:2152_2155_2174_2180clone_4-7-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR&lt;br&gt;<br /> | 2015 pEC2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + EcoR1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-9<br /> <br /> Make miniprep and digest with Nde1 + EcoR1&lt;br&gt;<br /> <br /> [[Image:2152_2155digest_4-9-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; [[Category:Cloning]]</div> Paul.xu