https://eln.excellgen.com/index.php?action=history&feed=atom&title=2156-pJ912-HIS4 2156-pJ912-HIS4 - Revision history 2026-10-18T16:39:50Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2156-pJ912-HIS4&diff=11817&oldid=prev Paul.xu at 15:57, 9 May 2014 2014-05-09T15:57:38Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2156-pJ912-HIS4&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2156-pJ912-HIS4 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-14--2014-4-23 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2156&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2156 2169pcr 4-10-2014.JPG]]&lt;br&gt; <br /> <br /> PCR HIS4 from 2158 pPIC9k&lt;br&gt;HIS4-BamH1-F&lt;br&gt;HIS4-ILV5-R&lt;br&gt;&lt;br&gt; <br /> <br /> 2. PCR ILV5 from 1842 pJ912-Irisin-1 <br /> <br /> HIS4-ILV5-F&lt;br&gt;ILV5-Nde1-R&lt;br&gt; <br /> <br /> [[Image:2148pcr 2156PCR 4-11-2014.JPG]]&lt;br&gt; <br /> <br /> pcr1+pcr2&lt;br&gt; <br /> <br /> [[Image:2148 2156 2172 2173clone 4-14-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pcr&lt;br&gt; <br /> | 1842 pJ912-Irisin-1&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | BamH1 + Nde1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-23 <br /> <br /> Make miniprep and digest with BamH1 + Nde1&lt;br&gt; <br /> <br /> [[Image:2143 2156 2168digest 4-23-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 2156-pJ912-HIS4&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2156-pJ912-HIS4 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-5-1---2014-5-7 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2156&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2156 2169pcr 4-10-2014.JPG]]&lt;br&gt; <br /> <br /> PCR HIS4 from 2158 pPIC9k&lt;br&gt;HIS4-BamH1-F&lt;br&gt;HIS4-ILV5-R&lt;br&gt;&lt;br&gt; <br /> <br /> 2. PCR ILV5 from 1842 pJ912-Irisin-1 <br /> <br /> HIS4-ILV5-F&lt;br&gt;ILV5-Nde1-R&lt;br&gt; <br /> <br /> [[Image:2156 2194pcr 5-7-2014.JPG]]&lt;br&gt; <br /> <br /> pcr1+pcr2&lt;br&gt; <br /> <br /> [[Image:2156clone 5-7-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | pcr&lt;br&gt; <br /> | 1842 pJ912-Irisin-1&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | BamH1 + Nde1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-5-7 <br /> <br /> Make miniprep and digest with BamH1 + Nde1/Nde1+Sal1&lt;br&gt; <br /> <br /> [[Image:2156digest 5-7-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> [[Image:2156-3pcr_screen_5-9-2014.JPG]]&lt;br&gt;<br /> <br /> 2156-3 pcr screen:AOX1-seq-F_R+HIS4-ILV5-R&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu