https://eln.excellgen.com/index.php?action=history&feed=atom&title=2157-pJ912-MS2Pac-iCre 2157-pJ912-MS2Pac-iCre - Revision history 2026-10-18T16:58:45Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2157-pJ912-MS2Pac-iCre&diff=11640&oldid=prev Paul.xu: Created page with "== '''Cloning of 2157-pJ912-MS2Pac-iCre''' == '''Purpose:''' Cloning of 2157-pJ912-MS2Pac-iCre '''Date: '''2014-4-3---2014-4-7 '''Notebook:0''' '''Page:''' '''Clone ID:2..." 2014-04-07T19:13:15Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2157-pJ912-MS2Pac-iCre&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2157-pJ912-MS2Pac-iCre &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-3---2014-4-7 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2157-pJ912-MS2Pac-iCre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2157-pJ912-MS2Pac-iCre <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-3---2014-4-7<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2157&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2155_2157_2159PCR_4-4-2014.JPG]]&lt;br&gt; <br /> <br /> PCR iCre from 1520 pDIRE&lt;br&gt;MS2Pac-NLS-EcoR1-F&lt;br&gt;iCre-MS2Pac-Not1-Xho1-R&lt;br&gt;<br /> <br /> [[Image:2157_2159clone_4-4-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR&lt;br&gt;<br /> | 1842 pJ912-Irisin-1&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | EcoR1 + Eag1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-7<br /> <br /> Make miniprep and digest with&amp;nbsp; EcoR1 + Xho1&lt;br&gt;<br /> <br /> [[Image:2157_2159digest_4-7-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu