https://eln.excellgen.com/index.php?action=history&feed=atom&title=2163-pT7A3E-HC-Tma-Endo-IV 2163-pT7A3E-HC-Tma-Endo-IV - Revision history 2026-10-18T16:06:31Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2163-pT7A3E-HC-Tma-Endo-IV&diff=11809&oldid=prev Paul.xu at 18:23, 7 May 2014 2014-05-07T18:23:49Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2163-pT7A3E-HC-Tma-Endo-IV&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2163-pT7A3E-HC-Tma-Endo-IV<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-24---2014-4-28<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2163&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning&lt;br&gt; ===<br /> <br /> [[Image:2186 2188 2163 2189 2190PCR 4-25-2014.JPG]]&lt;br&gt; <br /> <br /> PCR&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | 9&lt;br&gt; <br /> | 10&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR&lt;br&gt; <br /> | 1963 pT7A3E-HC&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2033 2085 2093 2101 2163clone 4-25-2014.JPG]]&lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-28<br /> <br /> Make miniprep and digest with&amp;nbsp; Nde1 + Xho1&lt;br&gt;<br /> <br /> [[Image:2163_2190digest_4-28-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu