https://eln.excellgen.com/index.php?action=history&feed=atom&title=2169-pPIC9k-Enterokinase 2169-pPIC9k-Enterokinase - Revision history 2026-10-18T14:13:31Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2169-pPIC9k-Enterokinase&diff=11772&oldid=prev Paul.xu: Created page with "== '''Cloning of 2169-pPIC9k-Enterokinase''' == '''Purpose:''' Cloning of 2169-pPIC9k-Enterokinase '''Date: '''2014-4-24---2014-4-28 '''Notebook:0''' '''Page:''' '''Clone..." 2014-04-30T18:42:45Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2169-pPIC9k-Enterokinase&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2169-pPIC9k-Enterokinase &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-24---2014-4-28 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2169-pPIC9k-Enterokinase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2169-pPIC9k-Enterokinase<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-24---2014-4-28<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2169&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2033_2093_2101_2169_2185PCR_4-25-2014.JPG]]<br /> <br /> PCR enterokinase from 1816 pCold-MBP-(E3C)-Enterokinase<br /> <br /> enterokinase-EcoR1-F C5 &lt;br&gt;C-6XHis-Nde1-Not1-R&lt;br&gt;<br /> <br /> [[Image:2169_2185_2186_2187_2188_2189clone_4-25-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pcr&lt;br&gt;<br /> | 2158 pPIC9k&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | EcoR1 + Not1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-28<br /> <br /> Make miniprep and digest with&amp;nbsp; EcoR1 + EcoRV<br /> <br /> [[Image:2169_2189digest_4-30-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu