https://eln.excellgen.com/index.php?action=history&feed=atom&title=2172-pCMV5-CD16-Tat-iCre-RIgE 2172-pCMV5-CD16-Tat-iCre-RIgE - Revision history 2026-10-18T18:30:36Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2172-pCMV5-CD16-Tat-iCre-RIgE&diff=11690&oldid=prev Paul.xu: Created page with "== '''Cloning of 2172-pCMV5-CD16-Tat-iCre-RIgE''' == '''Purpose:''' Cloning of 2172-pCMV5-CD16-Tat-iCre-RIgE '''Date: '''2014-4-14---2014-4-16 '''Notebook:0''' '''Page:'''..." 2014-04-16T17:48:31Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2172-pCMV5-CD16-Tat-iCre-RIgE&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2172-pCMV5-CD16-Tat-iCre-RIgE &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-14---2014-4-16 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2172-pCMV5-CD16-Tat-iCre-RIgE&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2172-pCMV5-CD16-Tat-iCre-RIgE <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-14---2014-4-16<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2172&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2148_2156_2172_2173clone_4-14-2014.JPG]]&lt;br&gt; <br /> <br /> subclone tat-Cre from 1972 pCMV5-Tat-Cre-PDGFR to 1973 pCMV5-CD16-RIgE using Kpn1 + Nhe1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-1-24 <br /> <br /> Make miniprep and digest with&amp;nbsp;Kpn1 + Nhe1&lt;br&gt;<br /> <br /> &amp;nbsp;[[Image:2172_2173digest_4-16-2014.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu