https://eln.excellgen.com/index.php?action=history&feed=atom&title=2180_2130_2174_cre_2108s_2108u 2180 2130 2174 cre 2108s 2108u - Revision history 2026-10-18T08:21:07Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2180_2130_2174_cre_2108s_2108u&diff=11680&oldid=prev Paul.xu: Created page with "Expression screening, 4/13/2014, by Shanyi<br> ? mM IPTG induction o/n at RT, 15 ml culture<br> Image:2180_2130_2174_cre_2108s_2108u.jpgg<br> {| width="747" height="540..." 2014-04-13T20:36:46Z <p>Created page with &quot;Expression screening, 4/13/2014, by Shanyi&lt;br&gt; ? mM IPTG induction o/n at RT, 15 ml culture&lt;br&gt; <a href="/index.php?title=File:2180_2130_2174_cre_2108s_2108u.jpg" title="File:2180 2130 2174 cre 2108s 2108u.jpg">Image:2180_2130_2174_cre_2108s_2108u.jpgg</a>&lt;br&gt; {| width=&quot;747&quot; height=&quot;540...&quot;</p> <p><b>New page</b></p><div>Expression screening, 4/13/2014, by Shanyi&lt;br&gt; <br /> <br /> ? mM IPTG induction o/n at RT, 15 ml culture&lt;br&gt;<br /> <br /> [[Image:2180_2130_2174_cre_2108s_2108u.jpg]]g&lt;br&gt;<br /> <br /> <br /> <br /> {| width=&quot;747&quot; height=&quot;540&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Competent Cells&lt;br&gt; <br /> | Sample&lt;br&gt; <br /> | Expression&lt;br&gt; <br /> | Note&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | 2180&lt;br&gt; <br /> | pT7A3-R9-HA-iFlp&lt;br&gt; <br /> | Nico(DE3)pLysN&lt;br&gt; <br /> | S&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | 2180&lt;br&gt; <br /> | &lt;br&gt; <br /> | Nico(DE3)pLysN&lt;br&gt; <br /> | P&lt;br&gt; <br /> | <br /> IB?9.65 /54.8<br /> <br /> | &lt;br&gt;<br /> |-<br /> | &#039;&#039;&#039;3&lt;br&gt;&#039;&#039;&#039; <br /> | 2180&lt;br&gt; <br /> | pT7A3-R9-HA-iFlp&lt;br&gt; <br /> | Tuner(DE3)pLysN&lt;br&gt; <br /> | S&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | 2180&lt;br&gt; <br /> | &lt;br&gt; <br /> | Tuner(DE3)pLysN&lt;br&gt; <br /> | P&lt;br&gt; <br /> | <br /> IB?9.65 /54.8&lt;br&gt;<br /> <br /> | &lt;br&gt;<br /> |-<br /> | M&lt;br&gt; <br /> | &lt;br&gt; <br /> | Fermentas&lt;br&gt; <br /> | PageRuler Plus&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | 2130&lt;br&gt; <br /> | pT7A3-6H-NLS-Cre&lt;br&gt; <br /> | Tuner(DE3)pLysN&lt;br&gt; <br /> | &lt;br&gt; <br /> | <br /> yes 9.87 /40.3&lt;br&gt;<br /> <br /> | &lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | 2130&lt;br&gt; <br /> | pT7A3-6H-NLS-Cre&lt;br&gt; <br /> | Nico(DE3)pLysN&lt;br&gt; <br /> | &lt;br&gt; <br /> | <br /> yes 9.87 /40.3&lt;br&gt;<br /> <br /> | &lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | 2174&lt;br&gt; <br /> | pCold-R9-HA-Cre&lt;br&gt; <br /> | Nico(DE3)pLysN&lt;br&gt; <br /> | &lt;br&gt; <br /> | <br /> low 10.09/44.7&lt;br&gt;<br /> <br /> | &lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | 2174&lt;br&gt; <br /> | pCold-R9-HA-Cre&lt;br&gt; <br /> | Tuner(DE3)pLysN&lt;br&gt; <br /> | &lt;br&gt; <br /> | No&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | &lt;br&gt; <br /> | Cre&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | 10.30 / 42.8&lt;br&gt; <br /> | size is a little smaller&lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | 2108&lt;br&gt; <br /> | pCold-Tat-NLS-iFLP-6H&lt;br&gt; <br /> | &lt;br&gt; <br /> | s&lt;br&gt; <br /> | 9.77 / 53.3&lt;br&gt; <br /> | size is a little larger&lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | 2108&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | p&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &#039;&#039;&#039;Lyse cell by freeze-thawing, 2 cycles, using cell lysis buffer, spin--&amp;gt; Supernatant (S)&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Add screening lysis buffer containing 4M urea, 1% SDS in Buffer A to pellet, solubilize and spin--&amp;gt;P&#039;&#039;&#039;&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu