https://eln.excellgen.com/index.php?action=history&feed=atom&title=2184_pMAL-c5E-R9-HA-iFlp 2184 pMAL-c5E-R9-HA-iFlp - Revision history 2026-10-18T23:05:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2184_pMAL-c5E-R9-HA-iFlp&diff=11788&oldid=prev Paul.xu: Created page with "== '''Cloning of 2184 pMAL-c5E-R9-HA-iFlp''' == '''Purpose:''' Cloning of2184 pMAL-c5E-R9-HA-iFlp'''==''' '''Date: '''2014-4---- '''Notebook:0''' '''Page:''' '''Clone I..." 2014-05-04T21:18:27Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2184 pMAL-c5E-R9-HA-iFlp&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2184 pMAL-c5E-R9-HA-iFlp&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone I...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2184 pMAL-c5E-R9-HA-iFlp&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2184 pMAL-c5E-R9-HA-iFlp&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2184&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> digest 2180 pT7A3-R9-HA-iFlp with Nco1 + Xho1, digest vector 1700 pMAL-c5E Nco1+ Sal1.<br /> <br /> [[Image:Digest_for_2178_2179_2184.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening&lt;br&gt; ===<br /> <br /> Date: 2014_4_ <br /> <br /> Make miniprep, digestion with HindIII + BamH1&lt;br&gt; <br /> <br /> [[Image:2178_2179_2184digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu