https://eln.excellgen.com/index.php?action=history&feed=atom&title=2185-pCold-MBP-%28Ent-TEV%29 2185-pCold-MBP-(Ent-TEV) - Revision history 2026-10-18T14:12:31Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2185-pCold-MBP-(Ent-TEV)&diff=11792&oldid=prev Paul.xu: Created page with "== '''Cloning of 2185-pCold-MBP-(Ent-TEV)''' == '''Purpose:''' Cloning of 2185-pCold-MBP-(Ent-TEV) '''Date: '''2014-5-2---2014-5-5 '''Notebook:0''' '''Page:''' '''Clone I..." 2014-05-05T19:55:34Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2185-pCold-MBP-(Ent-TEV)&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2185-pCold-MBP-(Ent-TEV) &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-5-2---2014-5-5 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone I...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2185-pCold-MBP-(Ent-TEV)&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2185-pCold-MBP-(Ent-TEV)<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-5-2---2014-5-5<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2185&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2093_2163_2185pcr_4-24-2014.JPG]]&lt;br&gt;<br /> <br /> PCR from 1804 pCold-MBP-(E3C)<br /> <br /> 1. Step 1&lt;br&gt;Fragment 1&lt;br&gt;Cold-promoter-Nhe1-F&lt;br&gt;Cold-pomoter-MBP-R&lt;br&gt;&lt;br&gt;<br /> <br /> Fragment 2<br /> <br /> Cold-pomoter-MBP-F&lt;br&gt;MBP-TEV-Nco1-Nde1-R&lt;br&gt;<br /> <br /> [[Image:2033_2093_2101_2169_2185PCR_4-25-2014.JPG]]<br /> <br /> PCR:pcr1+pcr2&lt;br&gt;<br /> <br /> [[Image:2169_2185_2186_2187_2188_2189clone_4-25-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR&lt;br&gt;<br /> | 1804 pCold-MBP-(E3C)&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nhe1 + Nde1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-5-5<br /> <br /> Make miniprep and digest with Nhe1 + Nco1<br /> <br /> [[Image:2185_2187digest_5-5-2014.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu