https://eln.excellgen.com/index.php?action=history&feed=atom&title=2186-pT7A3-HC-R9-HA 2186-pT7A3-HC-R9-HA - Revision history 2026-10-18T07:14:04Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2186-pT7A3-HC-R9-HA&diff=12563&oldid=prev Excellgen at 20:03, 28 July 2014 2014-07-28T20:03:51Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2186-pT7A3-HC-R9-HA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2186-pT7A3-HC-R9-HA <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-24---2014-4-28 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2186&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2186 2188 2163 2189 2190PCR 4-25-2014.JPG]] <br /> <br /> PCR: template2180&lt;br&gt; <br /> <br /> R9-HA-1 B10 &lt;br&gt;HA-Xho1-R&lt;br&gt; <br /> <br /> [[Image:2169 2185 2186 2187 2188 2189clone 4-25-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR&lt;br&gt; <br /> | 1885 pT7A3-HC&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nco1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-28 <br /> <br /> Colony screening by PCR using&amp;nbsp; pET-seq-F and &lt;br&gt;HA-Xho1-R&lt;br&gt; <br /> <br /> [[Image:2186pcr screen 4-28-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 2186-pT7A3-HC-R9-HA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2186-pT7A3-HC-R9-HA <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2186&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR: template2180&lt;br&gt; <br /> <br /> R9-HA-1 B10 &lt;br&gt;HA-Xho1-R&lt;br&gt; <br /> <br /> [[Image:2273 734 2249 1575-1 2272 2186pcr.JPG]]&lt;br&gt; <br /> <br /> digest with NcoI+XhoI, clone into 1885 pT7A3-HC&lt;br&gt; <br /> <br /> [[Image:Digest for 2238 2186 2272 2230.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_2_14 <br /> <br /> Make miniprep, use Nco1 + Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2186 2272digest.JPG]]&lt;br&gt; <br /> <br /> [[Image:2238 1577 1578 2186 2272 2012digest.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen