https://eln.excellgen.com/index.php?action=history&feed=atom&title=2189-pEC2-Tth-SOD 2189-pEC2-Tth-SOD - Revision history 2026-10-18T11:22:38Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2189-pEC2-Tth-SOD&diff=11777&oldid=prev Paul.xu at 19:44, 30 April 2014 2014-04-30T19:44:23Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2189-pEC2-Tth-SOD&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2189-pEC2-Tth-SOD <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-4-24---2014-4-28 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2189&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2186_2188_2163_2189_2190PCR_4-25-2014.JPG]]&lt;br&gt;<br /> <br /> PCR from Thermus thermophilus HB27&lt;br&gt;<br /> <br /> Tth-SOD-Nde1-F&lt;br&gt;Tth-SOD-EcoR1-R2&lt;br&gt;<br /> <br /> [[Image:2169_2185_2186_2187_2188_2189clone_4-25-2014.JPG]]&lt;br&gt;<br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR&lt;br&gt;<br /> | 2015 pEC2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | colspan=&quot;2&quot; | Nde1 + EcoR1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-4-28 <br /> <br /> Make miniprep and digest with Nde1 + EcoR1<br /> <br /> [[Image:2188_2189digest_4-28-2014.JPG]]&lt;br&gt;<br /> <br /> Make miniprep and digest with Xho1&lt;br&gt;<br /> <br /> [[Image:2169_2189digest_4-30-2014.JPG]]&lt;br&gt;<br /> <br /> clone 3 right<br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu