https://eln.excellgen.com/index.php?action=history&feed=atom&title=2195-pT7A3E-Vac-DNA-Ligase 2195-pT7A3E-Vac-DNA-Ligase - Revision history 2026-10-18T09:39:35Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2195-pT7A3E-Vac-DNA-Ligase&diff=11812&oldid=prev Paul.xu at 19:24, 8 May 2014 2014-05-08T19:24:03Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2195-pT7A3E-Vac-DNA-Ligase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2195-pT7A3E-Vac-DNA-Ligase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-5-5---2014-5-8<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2195&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:2049 2012 2195PCR 5-6-2014.JPG]]&lt;br&gt; <br /> <br /> template&amp;nbsp;:vaccine &lt;br&gt; <br /> <br /> Vac-DNAlig-R&lt;br&gt;Vac-DNAlig-F&lt;br&gt; <br /> <br /> [[Image:2195 2196clone 5-6-2014.JPG]]&lt;br&gt; <br /> <br /> {| width=&quot;200&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR&lt;br&gt; <br /> | 1892 pT7A3E&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 204-5-8 <br /> <br /> Make miniprep and digest with&amp;nbsp; Nde1 + Xho1 <br /> <br /> 2[[Image:2085_2195digest_5-8-2014.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu