https://eln.excellgen.com/index.php?action=history&feed=atom&title=2198_pT7A3E-Tth-RecA 2198 pT7A3E-Tth-RecA - Revision history 2026-10-18T11:50:57Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2198_pT7A3E-Tth-RecA&diff=13916&oldid=prev Excellgen at 21:36, 30 January 2015 2015-01-30T21:36:08Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2198 pT7A3E-Tth-RecA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2198 pT7A3E-Tth-RecA&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2198&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from HB8 genomic DNA using <br /> <br /> Tth-RecA-Nde1-F&lt;br&gt;tagctacatatggacgagagcaagcgcaag&lt;br&gt;Tth-RecA-EcoR1-R&lt;br&gt;(tggccgcgggcgaggacgagggggag CACCACCACCACCACCACTGA gaattc tacgac)&lt;br&gt;gtgctagaattcTCAGTGGTGGTGGTGGTGGTGccccttcatgctacccagggggag <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt;[[Image:212 2198 22292ndpcr.JPG]]&lt;br&gt; <br /> <br /> digest with Nde1 + EcoR1, clone to1982&lt;br&gt; <br /> <br /> [[Image:Digest for 2300 2214 2198 212.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8_ <br /> <br /> Make miniprep, use&amp;nbsp;Nde1 + EcoR1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:212 2198digest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Cloning of 2198 pT7A3E-Tth-RecA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2198 pT7A3E-Tth-RecA&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2015-1---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2198&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from HB8 genomic DNA using <br /> <br /> Tth-RecA-Nde1-F&lt;br&gt;tagctacatatggacgagagcaagcgcaag&lt;br&gt;Tth-RecA-EcoR1-R&lt;br&gt;(tggccgcgggcgaggacgagggggag CACCACCACCACCACCACTGA gaattc tacgac)&lt;br&gt;gtgctagaattcTCAGTGGTGGTGGTGGTGGTGccccttcatgctacccagggggag <br /> <br /> [[Image:2198 2401 2229pcr.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> digest with Nde1 + EcoR1, clone to1982&lt;br&gt; <br /> <br /> [[Image:Digest for 736fix3 2198 2229 2416.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2015_1_ <br /> <br /> Make miniprep, use&amp;nbsp;Nde1 + EcoR1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:736fix3 2198 2229digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen