https://eln.excellgen.com/index.php?action=history&feed=atom&title=2201_2213_2214_2215_2233Gateway_reaction_QC 2026-10-18T05:17:21Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2201_2213_2214_2215_2233Gateway_reaction_QC&diff=13066&oldid=prev 2014-09-11T17:07:20Z

<p>Created page with &quot;2014/09 Gateway reaction and transfection/expression small scale gateway reaction using GFP 393 &lt;br&gt; 2201 pCMV5-Dest-Avi-8H 2215 pCMV5-Dest-Avi-8H-BirA &lt;br&gt; 2233 pCMV5-Dest-...&quot;</p> <p><b>New page</b></p><div>2014/09 Gateway reaction and transfection/expression <br /> <br /> small scale gateway reaction using GFP 393 &lt;br&gt;<br /> <br /> 2201 pCMV5-Dest-Avi-8H<br /> <br /> 2215 pCMV5-Dest-Avi-8H-BirA &lt;br&gt;<br /> <br /> 2233 pCMV5-Dest-C-HA &lt;br&gt;<br /> <br /> 2213 pET30b-Dest-C-Avi-His &lt;br&gt;<br /> <br /> 2214 pET30b-Dest-Avi-8H-BirA <br /> <br /> &lt;br&gt; miniprep &lt;br&gt;<br /> <br /> 2201, 2215,2233 gateway reaction clones digest with BamH1 + EcoR1(GFP=&amp;gt;790bp, DEST=&amp;gt;941bp)&lt;br&gt;<br /> <br /> [[Image:2201_2215_2233GATEWAY.JPG]]&lt;br&gt;<br /> <br /> 2213,2214 gateway reaction clones digest with NcoI+KpnI(no NcoI site in 2213,2214) <br /> <br /> [[Image:2213_2214GATEWAY.JPG]]&lt;br&gt;<br /> <br /> transfect 2201, 2215,2233 gateway reaction clones to 293&lt;br&gt;<br /> <br /> [[Image:2201_2215_2233GATEWAYTRANSFECTION.JPG]]<br /> <br /> [[Category:QC]]</div>

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