https://eln.excellgen.com/index.php?action=history&feed=atom&title=2210-pT7A3E-ULP1 2210-pT7A3E-ULP1 - Revision history 2026-10-18T12:08:09Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2210-pT7A3E-ULP1&diff=12165&oldid=prev Excellgen at 20:36, 3 July 2014 2014-07-03T20:36:51Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2210-pT7A3E-ULP1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2210-pT7A3E-ULP1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6-20---2014-6-26 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2210&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; LIANGHUO <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from 2209 ZM552-ULP1&lt;br&gt;SUMO-P1-Nde1-F&lt;br&gt;tagca catatg ctt gtt cct gaa tta aat g&lt;br&gt;Sumo-Xho1-R&lt;br&gt;(gattttaaccgacgctttaaaactcgag tagcac)&lt;br&gt;gtgctactcgagttttaaagcgtcggttaaaatc&lt;br&gt;digest with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:1274 2210 2248 2197 PCR.JPG]] <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === 2210 DIGESTED ===<br /> <br /> [[Image:2246 1571 2249 2210 digested.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-6-26 <br /> <br /> Make miniprep and digest with Nde1 + XhoI <br /> <br /> [[Image:1571 1855 2197 2210 screening.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> SELECT CLONE5 FOR SEQUENCING&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Excellgen