https://eln.excellgen.com/index.php?action=history&feed=atom&title=2212-pT7A3E-PDI1 2212-pT7A3E-PDI1 - Revision history 2026-10-18T05:51:18Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2212-pT7A3E-PDI1&diff=12224&oldid=prev Excellgen: Created page with "== '''Cloning of 2212-pT7A3E-PDI1''' == '''Purpose:''' Cloning of 2212-pT7A3E-PDI1 '''Date: '''2014-7-01---2014-7-02 '''Notebook:0''' '''Page:''' '''Clone ID:2212''' ..." 2014-07-07T19:55:48Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2212-pT7A3E-PDI1&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2212-pT7A3E-PDI1 &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7-01---2014-7-02 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2212&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2212-pT7A3E-PDI1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2212-pT7A3E-PDI1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7-01---2014-7-02 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2212&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; LIANGHUO <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from 2208 pDONR221-PDI1<br /> <br /> yPDI-Nde1-F&lt;br&gt;tcgaacatATGAAGTTTTCTGCTGGTGC<br /> <br /> yPDI-Xho1-R&lt;br&gt;(GATGCCATTCACGATGAATTGctcgag tagca)&lt;br&gt;tgctactcgagCAATTCATCGTGAATGGCATC&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> PCR image?&lt;br&gt; &lt;br&gt; <br /> <br /> === 2212 DIGESTED ===<br /> <br /> [[Image:1535_1910_2212_2216_Digested.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-7-01 <br /> <br /> Make miniprep and digest with NdeI + XhoI <br /> <br /> [[Image:1274_1910_1535_2212_2230_Screening.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> SELECT CLONE2 FOR SEQUENCING&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Excellgen