https://eln.excellgen.com/index.php?action=history&feed=atom&title=2213_pET30b-Dest-C-Avi-His 2213 pET30b-Dest-C-Avi-His - Revision history 2026-10-18T12:55:26Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2213_pET30b-Dest-C-Avi-His&diff=12776&oldid=prev Excellgen: Created page with "== '''Cloning of 2213 pET30b-Dest-C-Avi-His''' == '''Purpose:''' Cloning of2213 pET30b-Dest-C-Avi-His'''==''' '''Date: '''2014-8---- '''Notebook:0''' '''Page:''' '''Clo..." 2014-08-08T19:09:13Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2213 pET30b-Dest-C-Avi-His&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2213 pET30b-Dest-C-Avi-His&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clo...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2213 pET30b-Dest-C-Avi-His&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2213 pET30b-Dest-C-Avi-His&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2213&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR gateway cassette from 1336 &lt;br&gt;<br /> <br /> 1 ul of 10 uM attR1-Fix-Nde1-F tagcac cat atg gaa TCAACAAGTTTGTACAAAAAAG&lt;br&gt; 1 ul of 1 uM attR2-AviTag-R2 ( ctttcttgtacaaagtggt GGCGGCCTGAACGACATCTTCGAGGCCCAGAAAATCGAGTGGCAC) GTGCCACTCGATTTTCTGGGCCTCGAAGATGTCGTTCAGGCCGCCaccactttgtacaagaaag <br /> <br /> 1 ul of 10 uM attR2-AviTag8H-Kpn1-EcoR1-R1 (CAGAAAATCGAGTGGCACGAG CAC CAC CAT CAT CAC CAT CAC CAC TGA GGTACC gaattc tacga) tcgtagaattcGGTACCTCAGTGGTGATGGTGATGATGGTGGTGCTCGTGCCACTCGATTTTCTG&lt;br&gt;<br /> <br /> [[Image:2201 2213 2233 2256pcr.JPG]]&lt;br&gt; <br /> <br /> digest with Nde1 + EcoR1, clone into Nde1 + EcoR1 digested vector 16 pET30b.Amp, &lt;br&gt;<br /> <br /> &lt;br&gt; [[Image:Digest_for_15782nd_2241_2213.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_7 <br /> <br /> Make miniprep, use Nde1 + EcoR1to find positive clone&lt;br&gt; <br /> <br /> [[Image:2304_2213_2297DIGEST.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Excellgen