https://eln.excellgen.com/index.php?action=history&feed=atom&title=2215_pCMV5-Dest-Avi-8H-BirA 2215 pCMV5-Dest-Avi-8H-BirA - Revision history 2026-10-18T14:44:43Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2215_pCMV5-Dest-Avi-8H-BirA&diff=12961&oldid=prev Excellgen at 16:43, 26 August 2014 2014-08-26T16:43:36Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2215 pCMV5-Dest-Avi-8H-BirA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2215 pCMV5-Dest-Avi-8H-BirA&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2215&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone gateway cassette from 2201 pCMV5-Dest-Avi-8H using BamH1 + EcoR1 TO2216 <br /> <br /> &lt;br&gt; [[Image:DIGEST FOR 2267 2215 2232 2233.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8_ <br /> <br /> Make miniprep, use&amp;nbsp;BamH1 + EcoR1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2215_2300_2234digest.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen