https://eln.excellgen.com/index.php?action=history&feed=atom&title=2216-pCMV5-BirA 2216-pCMV5-BirA - Revision history 2026-10-18T11:55:26Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2216-pCMV5-BirA&diff=12239&oldid=prev Excellgen: Created page with "== '''Cloning of 2216-pCMV5-BirA''' == '''Purpose:''' Cloning of 2216-pCMV5-BirA '''Date: '''2014-7-01---2014-7-02 '''Notebook:0''' '''Page:''' '''Clone ID:2216''' ''..." 2014-07-07T20:21:23Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2216-pCMV5-BirA&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2216-pCMV5-BirA &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7-01---2014-7-02 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2216&#039;&#039;&#039; &#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2216-pCMV5-BirA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2216-pCMV5-BirA <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7-01---2014-7-02 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2216&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; LIANGHUO <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from BL21 genomic DNA<br /> <br /> BirA-22nt-IRES-EcoR1-Xho1-F&lt;br&gt;g taa gaattc ctcgag TTTATAATTTCTTCTTCCAGAA GCCACC ATGAAGGATAACACCGTGCCAC<br /> <br /> &lt;br&gt;BirA-Kpn1-Not1-R&lt;br&gt;(CCTGCGTAGTGCAGAAAAATAA GGTACC GCGGCCGC tagcac)&lt;br&gt;gtgctaGCGGCCGCGGTACCTTATTTTTCTGCACTACGCAGG&lt;br&gt;<br /> <br /> <br /> <br /> [[Image:2215 2242 PCR.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === 2216 DIGESTED ===<br /> <br /> [[Image:2197_2216_2249_digested.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-7-02 <br /> <br /> Make miniprep and digest with Nde1 + XhoI <br /> <br /> [[Image:2216_screening.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> SELECT CLONE1 FOR SEQUENCING&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Excellgen