https://eln.excellgen.com/index.php?action=history&feed=atom&title=2224_pFastbacDual-p10-VSVG-M 2224 pFastbacDual-p10-VSVG-M - Revision history 2026-10-18T18:05:20Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2224_pFastbacDual-p10-VSVG-M&diff=11990&oldid=prev Paul.xu: Created page with "== '''Cloning of 2224 pFastbacDual-p10-VSVG-Me''' == '''Purpose:''' Cloning of 2224 pFastbacDual-p10-VSVG-M '''Date: '''2014-6 '''Notebook:0''' '''Page:''' '''Clone ID:..." 2014-06-12T19:18:42Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2224 pFastbacDual-p10-VSVG-Me&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2224 pFastbacDual-p10-VSVG-M &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2224 pFastbacDual-p10-VSVG-Me&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2224 pFastbacDual-p10-VSVG-M <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2224&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning&lt;br&gt; ===<br /> <br /> PCR VSVG from 505 pCMV-VSVG &lt;br&gt;<br /> <br /> 1st fragment VSVG-Nco1-F ctcatttccatggacatcac VSVG-dKpn1-R gtattttagggtGccattgattatggtg <br /> <br /> 2nd fragment VSVG-dKpn1-F caccataatcaat ggC acc ctaaaatac VSVG-Kpn1-R A4 gtgtaggtaccttactttccaagtcggttcatc <br /> <br /> [[Image:2227_2228_2224_2225pcr1.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> 3. Purify on gel 4. Join them by PCR <br /> <br /> [[Image:2224pcr2.JPG]]&lt;br&gt;<br /> <br /> 5. digest with Nco1 + Kpn1 &lt;br&gt;<br /> <br /> [[Image:Digest_for_2112_22243nd.JPG]]&lt;br&gt; 6. clone back to vector 2223 pFastbacDualM &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-6 <br /> <br /> mini prep and digest with Nco1 + Kpn1 <br /> <br /> [[Image:2224-1DIGEST.JPG]][[Image:2224_2230digest.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu