https://eln.excellgen.com/index.php?action=history&feed=atom&title=2226_pFastBac-Dual-VSVG-HIVGag-FLP 2226 pFastBac-Dual-VSVG-HIVGag-FLP - Revision history 2026-10-19T02:46:42Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2226_pFastBac-Dual-VSVG-HIVGag-FLP&diff=11906&oldid=prev Paul.xu: Created page with "== '''Cloning of 2226 pFastBac-Dual-VSVG-HIVGag-FLP''' == '''Purpose:''' Cloning of 2226 pFastBac-Dual-VSVG-HIVGag-FLP'''==''' '''Date: '''2014-5---- '''Notebook:0''' '''..." 2014-05-22T15:44:24Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2226 pFastBac-Dual-VSVG-HIVGag-FLP&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2226 pFastBac-Dual-VSVG-HIVGag-FLP&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-5---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2226 pFastBac-Dual-VSVG-HIVGag-FLP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2226 pFastBac-Dual-VSVG-HIVGag-FLP&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-5---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2226&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone HIVGag-Flp from 1969 pCMV5-Gag-p2b-NLS-Flp-WPRE-oriP using BamH1 + Xba1TO 2100 pFastBac-Dual-VSVG <br /> <br /> [[Image:Digest for 2226 2223 2220.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014 <br /> <br /> Make miniprep, digest with HindIII <br /> <br /> [[Image:2226digest1.JPG]]&lt;br&gt; BamH1 + Xba1 to verify positive clone <br /> <br /> [[Image:2220_2226DIGEST.JPG]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu