https://eln.excellgen.com/index.php?action=history&feed=atom&title=2232_pT7A3-SortaseAM-C6H 2026-10-18T13:40:43Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2232_pT7A3-SortaseAM-C6H&diff=12959&oldid=prev 2014-08-26T16:41:47Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2232 pT7A3-SortaseAM-C6H&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2232 pT7A3-SortaseAM-C6H&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2232&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR using 2230 pT7A3-SortaseAM-N6H as template <br /> <br /> 1 ul of 10 uM SortaseA-Nde1-F1 tcgaacatatgGGCAGCAGCctcgagcttccggaaaccggaggaggtgggtctggaggtggaggatctggt 1 ul of 1 uM SortaseA-Nde1-F2 cggaaaccggaggaggtgggtctggaggtggaggatctggtggaggtgggtctCAAGCTAAACCTCAAATTCCG <br /> <br /> 1 ul of 10 uM SortaseA-Sal1-R tgctagtcgaccttgacttcAgtagctacaaag <br /> <br /> [[Image:2267 2232pcr.JPG]] <br /> <br /> digest with Nde1 + Sal1. Digest vector with Nde1 + Xho1 <br /> <br /> [[Image:DIGEST FOR 2267 2215 2232 2233.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8_ <br /> <br /> Make miniprep, use NdeI+HindIII to find positive clone&lt;br&gt; <br /> <br /> [[Image:2214_2232_2234digest.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

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