https://eln.excellgen.com/index.php?action=history&feed=atom&title=2238_pT7A3-uvrD 2238 pT7A3-uvrD - Revision history 2026-10-18T09:25:48Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2238_pT7A3-uvrD&diff=13788&oldid=prev Excellgen at 15:23, 31 December 2014 2014-12-31T15:23:38Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2238 pT7A3-uvrD&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2238 pT7A3-uvrD&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2238&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from MG1655 &lt;br&gt; <br /> <br /> [[Image:1577 1578 2238 1535 1855pcr.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; digest with Nco1 + XhoI, clone to 1279&lt;br&gt; <br /> <br /> [[Image:Digest for 2238 2186 2272 2230.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_7 <br /> <br /> Make miniprep, use Nco1 + XhoI to find positive clone&lt;br&gt; <br /> <br /> [[Image:2238 1577 1578 2186 2272 2012digest.JPG]] <br /> <br /> use NdeI+BamHI to verify <br /> <br /> [[Image:2238DIGEST.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> sent 2238-1to sequence, maybe missing two nt&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Cloning of 2238 pEC2-uvrD&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2238 pEC2-uvrD&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-12---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2238&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from MG1655 &lt;br&gt; <br /> <br /> [[Image:2256_2238_1578pcr.JPG]]&lt;br&gt; &lt;br&gt; digest with Nco1 + XhoI, clone to 2015&lt;br&gt; <br /> <br /> [[Image:Digest_for_2238a_2201_2233.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_7 <br /> <br /> Make miniprep, use Nco1 + XhoI to find positive clone&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen