https://eln.excellgen.com/index.php?action=history&feed=atom&title=2244-pT7A3E-pelB-Lambda-Exo 2244-pT7A3E-pelB-Lambda-Exo - Revision history 2026-10-18T13:42:57Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2244-pT7A3E-pelB-Lambda-Exo&diff=12221&oldid=prev Excellgen: Created page with "== '''Cloning of 2244-pT7A3E-pelB-Lambda-Exo''' == '''Purpose:''' Cloning of 2244-pT7A3E-pelB-Lambda-Exo '''Date: '''2014-7-01---2014-7-02 '''Notebook:0''' '''Page:''' ..." 2014-07-07T19:35:56Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2244-pT7A3E-pelB-Lambda-Exo&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2244-pT7A3E-pelB-Lambda-Exo &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7-01---2014-7-02 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2244-pT7A3E-pelB-Lambda-Exo&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2244-pT7A3E-pelB-Lambda-Exo <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7-01---2014-7-02 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2244&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; LIANGHUO <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from lambda DNA or clone 1026 pET28b-LambdaExo <br /> <br /> 1 ul of 10 uM pelB-Xba1-F&lt;br&gt;tagcTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAATGAAATACCTGCTGCCGACCGCTGCtGCTG <br /> <br /> &lt;br&gt;1 ul of 1 uM pelB-lambdaExo-F&lt;br&gt;CTGCTGCCGACCGCTGCtGCTGGTCTgCTGCTCCTCGCTGCCCAGCCGGCGATGGCCcatatgacaccggacattatcctgcag <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt;1 ul of 10 uM LambdaExo-Xho1-R2 <br /> <br /> (gtatttggggagcaatggcga ctcgagcgatct)&lt;br&gt;agatcgctcgagtcgccattgctccccaaatac&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2244 2245 2246 2247 PCR.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> === 2244 DIGESTED ===<br /> <br /> [[Image:2244 2245 digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-7-01 <br /> <br /> Make miniprep and digest with XbaI + XhoI <br /> <br /> [[Image:2244 2245 screening.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> SELECT CLONE6 FOR SEQUENCING&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Excellgen