https://eln.excellgen.com/index.php?action=history&feed=atom&title=2246-pT7A3E-Lambda-Xis 2246-pT7A3E-Lambda-Xis - Revision history 2026-10-18T09:39:22Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2246-pT7A3E-Lambda-Xis&diff=12167&oldid=prev Excellgen: Created page with "== '''Cloning of 2246-pT7A3E-Lambda-Xis''' == '''Purpose:''' Cloning of 2246-pT7A3E-Lambda-Xis '''Date: '''2014-6-24---2014-6-26 '''Notebook:0''' '''Page:''' '''Clone I..." 2014-07-03T20:51:54Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2246-pT7A3E-Lambda-Xis&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2246-pT7A3E-Lambda-Xis &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6-24---2014-6-26 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone I...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2246-pT7A3E-Lambda-Xis&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2246-pT7A3E-Lambda-Xis <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6-24---2014-6-26 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2246&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; LIANGHUO <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from 1149 pT7A3E-6H-HC-Xis or 1186 pDB.His.MBP-Xis<br /> <br /> Xis-Nde1-F&lt;br&gt;tagca ctagc cat atgtacttgacacttcaggag<br /> <br /> Xis-Xho1-F&lt;br&gt;(gaaatgggaagaaggcgaagtca ctcgag tagca)&lt;br&gt;tgctactcgagtgacttcgccttcttcccatttc<br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2244_2245_2246_2247_PCR.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; 2246 DIGESTED <br /> <br /> [[Image:2246 1571 2249 2210 digested.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-6-26 <br /> <br /> Make miniprep and digest with Nde1 + XhoI <br /> <br /> [[Image:2210_2246_2248_screening.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> SELECT CLONE1 FOR SEQUENCING&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Excellgen