https://eln.excellgen.com/index.php?action=history&feed=atom&title=2248-pT7A3E-UDG 2248-pT7A3E-UDG - Revision history 2026-10-18T08:47:10Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2248-pT7A3E-UDG&diff=12213&oldid=prev Excellgen: Created page with "== '''Cloning of 2248-pT7A3E-UDG''' == '''Purpose:''' Cloning of 2248-pT7A3E-ULP1 '''Date: '''2014-6-20---2014-6-26 '''Notebook:0''' '''Page:''' '''Clone ID:2248''' '..." 2014-07-07T19:16:17Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2248-pT7A3E-UDG&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2248-pT7A3E-ULP1 &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6-20---2014-6-26 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2248&#039;&#039;&#039; &#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2248-pT7A3E-UDG&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2248-pT7A3E-ULP1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-6-20---2014-6-26 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2248&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; LIANGHUO <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> &lt;br&gt;<br /> <br /> PCR using <br /> <br /> &lt;br&gt;UDG-Nde1-F&lt;br&gt;tacaccatATGGAACTGTTTGATGAACAG<br /> <br /> &lt;br&gt;UDG-Xho1-R&lt;br&gt;(GATTGATTGGCAACTGCCGCAG ctcgag tatag)&lt;br&gt; <br /> <br /> [[Image:1274 2210 2248 2197 PCR.JPG]] <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === 2248 DIGESTED ===<br /> <br /> [[Image:2248_2197_Digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014-6-26 <br /> <br /> Make miniprep and digest with Nde1 + XhoI <br /> <br /> [[Image:2210_2246_2248_screening.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> SELECT CLONE1 FOR SEQUENCING&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Excellgen