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224 PLenti-cfp - Revision history
2026-10-18T09:26:00Z
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Hylei: Created page with "== '''SubCloning of CFP to pLentiplus''' == '''Purpose:''' Subcloning of cfp to pLentiplus from pAAV-CFP. '''Date: '''2011-8-15 '''Notebook:''' hAIYAN '''Page:''' 003019 ..."
2011-08-15T18:21:22Z
<p>Created page with "== '''SubCloning of CFP to pLentiplus''' == '''Purpose:''' Subcloning of cfp to pLentiplus from pAAV-CFP. '''Date: '''2011-8-15 '''Notebook:''' hAIYAN '''Page:''' 003019 ..."</p>
<p><b>New page</b></p><div>== '''SubCloning of CFP to pLentiplus''' ==<br />
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'''Purpose:''' Subcloning of cfp to pLentiplus from pAAV-CFP. <br />
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'''Date: '''2011-8-15 <br />
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'''Notebook:''' hAIYAN <br />
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'''Page:''' 003019 <br />
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'''Clone ID:''' 224 <br />
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'''Experimenters:''' Haiyan <br />
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== '''Results''' ==<br />
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=== Subcloning ===<br />
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Date: 2011-8-15<br> <br />
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Digest pAAV-CFP with BamH1 + Xho1<br> <br />
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Run gel and cut out insert <br />
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Digest pLentiPlus with BamH1 + Xho1 <br />
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Run gel and cut out vector combine and purify using spin column <br />
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Ligate and transform STBL3 <br />
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=== Colony Screening ===<br />
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Date: 2011-8-15 <br />
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Make miniprep and digest pLenti-CFP with BamHI + Xho1 <br />
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<br> [[Image:IMG_1269.JPG]] <br />
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Lanes 6-9: pLenti-CFP clone 1-4, BamHI + Xho1<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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*clone 1,2,3 should be positive clone<br><br />
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== '''Conclusion''' ==<br />
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finish clone 224<br> <br />
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=== ===<br />
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[[User:Haiyan]] 11:33, 08/15/2011 (EST)<br> <br />
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[[Category:Cloning]]</div>
Hylei