https://eln.excellgen.com/index.php?action=history&feed=atom&title=2255_pT7A3-wtSM2-b 2255 pT7A3-wtSM2-b - Revision history 2026-10-18T17:21:40Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2255_pT7A3-wtSM2-b&diff=12699&oldid=prev Excellgen at 21:40, 1 August 2014 2014-08-01T21:40:05Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2255 pT7A3-wtSM2-b&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2255 pT7A3-wtSM2-b&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7--2014/8/1-- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2255&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR using new oligos, 1680 pCold-GST-wtSM2 as template <br /> <br /> 1 ul of 10 uM SM2-signal-Xba1-F1 tagcTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAATGCGCTTTAACAACAAGAATGTTGGCCTTG &lt;br&gt; <br /> <br /> 1 ul of 1 uM SM2-signal-Nde1-F2 ACAAGAATGTTGGCCTTGGCGCCCTGCTGTTCGCCGCGCAGGCGTCGGCCgaccatatgacgctcgaatccatcgac <br /> <br /> 1 ul of 10 uM WtSM2-Xho1-R (gagttgatgggctgcaaaaac taa ctcgag ctagca) tgctagctcgagttagtttttgcagcccatcaactc <br /> <br /> [[Image:2273-2 2286 2254 2255pcr.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; digest with Xba1 + Xho1, clone to 1727 pT7A30&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2255 1575.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8_1 <br /> <br /> Make miniprep, use Xba1 + Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2287 2255 2291digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen