https://eln.excellgen.com/index.php?action=history&feed=atom&title=2267_pT7A3-Tth-PPase 2267 pT7A3-Tth-PPase - Revision history 2026-10-18T17:24:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2267_pT7A3-Tth-PPase&diff=13080&oldid=prev Excellgen at 18:28, 12 September 2014 2014-09-12T18:28:55Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2267 pT7A3-Tth-PPase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2267 pT7A3-Tth-PPase&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2267&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from Thermus thermophilus HB27 genomic DNA <br /> <br /> &lt;br&gt; <br /> <br /> Fragment 1&lt;br&gt;Tth-PPase-Nde1-F <br /> <br /> atcgac catatggcgaacctgaagagccttc&lt;br&gt;2267-Tth-PPas-R1 cttggcctcCagggccttg <br /> <br /> &lt;br&gt;Fragment 2&lt;br&gt;2267-Tth-PPas-F1 caaggccctGgaggccaag&lt;br&gt;Tth-PPase-Xho1-R&lt;br&gt;gtgctactcgaggcccttgtagcgggcgatgcag&lt;br&gt;(ctgcatcgcccgctacaagggc ctcgag tagcac) <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2267PCR1ST.JPG]]&lt;br&gt; <br /> <br /> Join 2 fragments <br /> <br /> Tth-PPase-Nde1-F <br /> <br /> atcgac catatggcgaacctgaagagccttc <br /> <br /> Tth-PPase-Xho1-R&lt;br&gt;gtgctactcgaggcccttgtagcgggcgatgcag&lt;br&gt;(ctgcatcgcccgctacaagggc ctcgag tagcac <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2166 2302 2267PCR.JPG]]&lt;br&gt; digest with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2166 2302 2267 2336PCR.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; Nde1 + Xho1to find positive clone&lt;br&gt; <br /> <br /> [[Image:2166 2267 2336DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen