https://eln.excellgen.com/index.php?action=history&feed=atom&title=2272_pEC2Bsu 2272 pEC2Bsu - Revision history 2026-10-18T10:47:49Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2272_pEC2Bsu&diff=12561&oldid=prev Excellgen at 20:00, 28 July 2014 2014-07-28T20:00:21Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2272 pEC2Bsu&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2272 pEC2Bsu&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2272&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> gene synthesis of MCS <br /> <br /> [[Image:2272pcr.JPG]]&lt;br&gt; <br /> <br /> digest with Nco1 + Xba1, clone to 2015pEC2&lt;br&gt; <br /> <br /> [[Image:Digest for 2238 2186 2272 2230.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_7_16 <br /> <br /> Make miniprep, use Nco1 + Xba1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2186 2272digest.JPG]]&lt;br&gt; <br /> <br /> [[Image:2238 1577 1578 2186 2272 2012digest.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen