https://eln.excellgen.com/index.php?action=history&feed=atom&title=2279_pLIKE-rep-T7-Endo1 2279 pLIKE-rep-T7-Endo1 - Revision history 2026-10-18T16:40:06Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2279_pLIKE-rep-T7-Endo1&diff=12570&oldid=prev Excellgen at 20:12, 28 July 2014 2014-07-28T20:12:50Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2279 pLIKE-rep-T7-Endo1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2279 pLIKE-rep-T7-Endo1&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2279&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 1225 pET28b-T7-Endo1 &lt;br&gt; <br /> <br /> T7-endo1-Xba1-F tacta tctaga catatgg caggttacgg cgctaaag <br /> <br /> T7-endo1-HindIII-R (taaaaaggaa aggaggaaag aaa ctcgag GGT ACC CACCACCACCACCACCACTGA aagctt tagca) tgctaaagcttTCAGTGGTGGTGGTGGTGGTGGGTACCctcgagtttctttcctcctttccttttta <br /> <br /> [[Image:2264 734 2279 2273 2268 2278-2pcr.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> digest with Xba1 +HindIII, clone to 2270 pLIKE-rep&lt;br&gt; <br /> <br /> [[Image:Digest for 734 2279 2264 2015.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_2_14 <br /> <br /> Make miniprep, use&amp;nbsp; Xba1 +HindIII to find positive clone&lt;br&gt; <br /> <br /> [[Image:734_2279digest.JPG]]&lt;br&gt; <br /> <br /> SEQUENCED NOT RIGHT<br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen