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2286 pCMV5-SBGFP - Revision history
2026-10-18T12:56:59Z
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Paul.xu at 22:15, 10 August 2014
2014-08-10T22:15:46Z
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<p><b>New page</b></p><div>== '''Cloning of 2286 pCMV5-SBGFP''' ==<br />
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'''Purpose:''' Cloning of2286 pCMV5-SBGFP'''==''' <br />
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'''Date: '''2014-7--2014/8/1-- <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:2286''' <br />
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'''Experimenters:'''&nbsp; shanyi <br />
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== '''Results''' ==<br />
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=== CLONING ===<br />
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gene synthesis <br />
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[[Image:2273-2 2286 2254 2255pcr.JPG]]<br> <br />
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digest with NheI+KpnI,clone into 1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP<br> <br />
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[[Image:Digest for 734A 2279A 2286 2254.JPG]]<br> <br />
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=== Colony Screening ===<br />
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Date: 2014_8_1 <br />
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Make miniprep, use NheI+KpnI to find positive clone<br> <br />
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[[Image:2273 2286digest.JPG]] <br />
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digest with NcoI <br />
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[[Image:2273 2286 1395 2254 2287digest.JPG]]<br> <br />
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8/10/14<br> <br />
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Transfection clone #2, #4 and #5&nbsp; (row 3) are quite bright although frame shift<br />
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[[Image:2286_pCMV5-SBGFP.JPG]]<br />
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== '''Result intepretation/Summary''' ==<br />
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*<br><br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu