https://eln.excellgen.com/index.php?action=history&feed=atom&title=2287_pL6H-5H-Tat-5H-sso7d 2287 pL6H-5H-Tat-5H-sso7d - Revision history 2026-10-18T16:06:40Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2287_pL6H-5H-Tat-5H-sso7d&diff=12738&oldid=prev Excellgen: Created page with "== '''Cloning of 2287 pL6H-5H-Tat-5H-sso7d''' == '''Purpose:''' Cloning of2287 pL6H-5H-Tat-5H-sso7d'''==''' '''Date: '''2014-7--2014/8/1-- '''Notebook:0''' '''Page:''' ..." 2014-08-05T16:38:57Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2287 pL6H-5H-Tat-5H-sso7d&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2287 pL6H-5H-Tat-5H-sso7d&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7--2014/8/1-- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2287 pL6H-5H-Tat-5H-sso7d&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2287 pL6H-5H-Tat-5H-sso7d&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7--2014/8/1-- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2287&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR using 1847 pT7A30-sso7d-6H as template &lt;br&gt;<br /> <br /> 1 ul of 10 uM 2287-6H-Tat-5H-Nco1-F1 ATC ACC CAT GGG CAG CAG CCA TCA TCA TCA TCA TCA CGG TCG CAA GAA ACG TCG CCA ACG TCG CCG TCC GCC TGC AGG C <br /> <br /> 1 ul of 1 uM 2287-6H-Tat-5H-F2 TCG CCG TCC GCC TGC AGG CCA CCA TCA CCA TCA CGG TAC CGG TAG CGG CGG TGG TGG CGC CAC GGT CAA ATT CAA GTA C <br /> <br /> 1 ul of 10 uM 2287-sso7d-Xho1-R TGA TAC TCG AGT TAG CAT TTT TTC TGC TTC TCA AGC ATT TG <br /> <br /> [[Image:2273_2287pcr.JPG]]&lt;br&gt; <br /> <br /> digest with Nco1 +Xho1,clone into 1997 pL6H&lt;br&gt; <br /> <br /> [[Image:DIGEST_FOR_2273_2287.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8_1 <br /> <br /> Make miniprep, use Nco1 +Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2287_2255_2291digest.JPG]]&lt;br&gt; <br /> <br /> digest with NcoI+KpnI,will get a 94bp band&lt;br&gt;<br /> <br /> [[Image:2273_2286_1395_2254_2287digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen