https://eln.excellgen.com/index.php?action=history&feed=atom&title=2292_2295_2296_2044_2312_2301 2292 2295 2296 2044 2312 2301 - Revision history 2026-10-18T02:17:38Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2292_2295_2296_2044_2312_2301&diff=13029&oldid=prev Excellgen: Created page with "== Protein Expression == 2014/9/5<br> Image:2292_2295_2296_2044_2312_2301.jpg<br> {| width="832" cellspacing="1" cellpadding="1" border="1" |- | Lane<br> | Name<br> |..." 2014-09-08T16:08:58Z <p>Created page with &quot;== Protein Expression == 2014/9/5&lt;br&gt; <a href="/index.php?title=File:2292_2295_2296_2044_2312_2301.jpg" title="File:2292 2295 2296 2044 2312 2301.jpg">Image:2292_2295_2296_2044_2312_2301.jpg</a>&lt;br&gt; {| width=&quot;832&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; |- | Lane&lt;br&gt; | Name&lt;br&gt; |...&quot;</p> <p><b>New page</b></p><div>== Protein Expression ==<br /> <br /> 2014/9/5&lt;br&gt; <br /> <br /> [[Image:2292_2295_2296_2044_2312_2301.jpg]]&lt;br&gt; <br /> <br /> {| width=&quot;832&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | SAMPLE&lt;br&gt; <br /> | pI/Mw&lt;br&gt; <br /> | express&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | pT7A3E-yPolyA-Pol&lt;br&gt; <br /> | &lt;br&gt; <br /> | <br /> 2292&lt;br&gt; <br /> <br /> | <br /> 15 ml, <br /> <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | &amp;nbsp; 0.2mM IPTG RT O/N&lt;br&gt; <br /> | s&lt;br&gt; <br /> | <br /> 7.63/66.2&lt;br&gt; <br /> <br /> | yes&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | pET30b-yPolyA-Pol&lt;br&gt; <br /> | &lt;br&gt; <br /> | 2295&lt;br&gt; <br /> | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | 0.4mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | 7.63/66.2&lt;br&gt; <br /> | yes&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | pET28b-Exo-VII&lt;br&gt; &lt;br&gt; <br /> | &lt;br&gt; &lt;br&gt; <br /> | &lt;br&gt; 2296&lt;br&gt; <br /> | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | 0.4mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | 9.94/52.9&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | pageRuler Plus Prestained protein ladder&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> | &lt;br&gt;&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | rowspan=&quot;2&quot; | pBad24-Tat-yFLP &lt;br&gt; &amp;nbsp; &lt;br&gt;<br /> | rowspan=&quot;2&quot; | &lt;br&gt; &lt;br&gt;<br /> | rowspan=&quot;2&quot; | 2044 &lt;br&gt; &lt;br&gt;<br /> | 15 ml,<br /> TOP10plysN&lt;br&gt;<br /> <br /> | &lt;br&gt;10mM Arabinose RT O/N&lt;br&gt; <br /> | s&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | 10mM Arabinose RT O/N&lt;br&gt; <br /> | s&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | rowspan=&quot;3&quot; | pEB-iFLP&lt;br&gt; &lt;br&gt; &lt;br&gt;<br /> | rowspan=&quot;3&quot; | &lt;br&gt; &lt;br&gt; &amp;nbsp; &lt;br&gt;<br /> | rowspan=&quot;3&quot; | 2312 &lt;br&gt; &lt;br&gt; &lt;br&gt;<br /> | 15 ml, <br /> TOP10plysN&lt;br&gt; <br /> <br /> | 10mM Arabinose RT O/N&lt;br&gt; <br /> | s&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | 10mM Arabinose RT O/N&lt;br&gt; <br /> | s&lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | <br /> &amp;nbsp;15 ml, <br /> <br /> Tuner(DE3)plysN &lt;br&gt; <br /> <br /> | 10mM Arabinose RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | rowspan=&quot;2&quot; | pR-wtSM2-NucA&lt;br&gt; &lt;br&gt;<br /> | rowspan=&quot;2&quot; | &lt;br&gt; &lt;br&gt;<br /> | rowspan=&quot;2&quot; | 2301 &lt;br&gt; &lt;br&gt;<br /> | rowspan=&quot;2&quot; | Ecoli K12 JM101&lt;br&gt;<br /> | rowspan=&quot;2&quot; | QHP&lt;br&gt;<br /> | concentrated&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 12&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Excellgen