https://eln.excellgen.com/index.php?action=history&feed=atom&title=2297_pEC-NotI 2297 pEC-NotI - Revision history 2026-10-18T23:05:30Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2297_pEC-NotI&diff=13333&oldid=prev Excellgen: Created page with "== '''Cloning of 2297 pEC-NotI''' == '''Purpose:''' Cloning of2297 pEC-NotI'''==''' '''Date: '''2014-9 '''Notebook:0''' '''Page:''' '''Clone ID:2297''' '''Experimente..." 2014-10-03T17:09:41Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2297 pEC-NotI&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2297 pEC-NotI&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2297&#039;&#039;&#039; &#039;&#039;&#039;Experimente...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2297 pEC-NotI&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2297 pEC-NotI&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2297&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 567 &lt;br&gt; <br /> <br /> Not1-Xba1-F atacactctagaTGCGTAGCGATACCAGCGTTG <br /> <br /> 2297-Not1-HindIII-R2 tgataAAGCTTTCAGTGGTGGTGGTGGTGGTGTGCATCAAACAGACCACGCTG <br /> <br /> [[Image:2297pcr.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; digest with Xba1 + HindIII, clone to1454 pEC&lt;br&gt; <br /> <br /> [[Image:Digest_for_2297_2321_2334_2335_2349_2348.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8_8 <br /> <br /> Make miniprep, use Xba1 + HindIII to find positive clone&lt;br&gt; <br /> <br /> [[Image:2297_22321_2335_2348DIGEST-.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen