https://eln.excellgen.com/index.php?action=history&feed=atom&title=2297_pEC2-NotI 2297 pEC2-NotI - Revision history 2026-10-18T17:18:40Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2297_pEC2-NotI&diff=13329&oldid=prev Excellgen at 17:04, 3 October 2014 2014-10-03T17:04:59Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2297 pEC2-NotI&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2297 pEC2-NotI&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7--2014/8/1-- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2297&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 567 &lt;br&gt; <br /> <br /> Not1-Xba1-F atacactctagaTGCGTAGCGATACCAGCGTTG <br /> <br /> Not1-HindIII-R (CAGCGTGGTCTGTTTGATGCA CACCACCACCACCACCACTGA AAGCTT) AAGCTTTCAGTGGTGGTGGTGGTGGTGTGCATCAAACAGACCACGCTG <br /> <br /> &lt;br&gt; [[Image:2264 734 2279 2273 2268 2278-2pcr.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; digest with Xba1 + HindIII, clone to 2015 pEC2&lt;br&gt; <br /> <br /> [[Image:Digest for 734A 2279A 2286 2254.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8_1 <br /> <br /> Make miniprep, use Xba1 + HindIII to find positive clone&lt;br&gt; <br /> <br /> [[Image:2297 2298digest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 2297 pEC2-NotI&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2297 pEC2-NotI&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2297&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 567 &lt;br&gt; <br /> <br /> Not1-Xba1-F atacactctagaTGCGTAGCGATACCAGCGTTG <br /> <br /> Not1-HindIII-R (CAGCGTGGTCTGTTTGATGCA CACCACCACCACCACCACTGA AAGCTT) AAGCTTTCAGTGGTGGTGGTGGTGGTGTGCATCAAACAGACCACGCTG <br /> <br /> &lt;br&gt; [[Image:2297 2012fixpcr.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; digest with Xba1 + HindIII, clone to 2015 pEC2&lt;br&gt; <br /> <br /> [[Image:Digest for 2297 2279.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8_8 <br /> <br /> Make miniprep, use Xba1 + HindIII to find positive clone&lt;br&gt; <br /> <br /> [[Image:2304 2213 2297DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> there are two HindIII site in the 2015 vector, so cannot use pEC2 to do this clone, use 1454pEC instead.&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen