https://eln.excellgen.com/index.php?action=history&feed=atom&title=2298_pEC2-T7-Endo1 2298 pEC2-T7-Endo1 - Revision history 2026-10-18T15:05:47Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2298_pEC2-T7-Endo1&diff=12740&oldid=prev Excellgen: Created page with "== '''Cloning of 2298 pEC2-T7-Endo1''' == '''Purpose:''' Cloning of2298 pEC2-T7-Endo1'''==''' '''Date: '''2014-7--2014/8/1-- '''Notebook:0''' '''Page:''' '''Clone ID:22..." 2014-08-05T16:41:32Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2298 pEC2-T7-Endo1&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2298 pEC2-T7-Endo1&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7--2014/8/1-- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:22...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2298 pEC2-T7-Endo1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2298 pEC2-T7-Endo1&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-7--2014/8/1-- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2298&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 1225 pET28b-T7-Endo1 <br /> <br /> T7-endo1-Xba1-F tacta tctaga catatgg caggttacgg cgctaaag <br /> <br /> T7-endo1-HindIII-R (taaaaaggaa aggaggaaag aaa ctcgag GGT ACC CACCACCACCACCACCACTGA aagctt tagca) tgctaaagcttTCAGTGGTGGTGGTGGTGGTGGGTACCctcgagtttctttcctcctttccttttta <br /> <br /> 2279pcr&lt;br&gt; [[Image:2264 734 2279 2273 2268 2278-2pcr.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; digest with Xba1 + HindIII, clone to 2015 pEC2&lt;br&gt; <br /> <br /> [[Image:Digest for 734A 2279A 2286 2254.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8_1 <br /> <br /> Make miniprep, use Xba1 + HindIII to find positive clone&lt;br&gt; <br /> <br /> [[Image:2297 2298digest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen