https://eln.excellgen.com/index.php?action=history&feed=atom&title=2302_pSBGFP 2302 pSBGFP - Revision history 2026-10-18T10:17:40Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2302_pSBGFP&diff=13098&oldid=prev Paul.xu at 02:10, 15 September 2014 2014-09-15T02:10:55Z <p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Cloning of 2302 pSBGFP&#039;&#039;&#039;&amp;nbsp; <br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2302 pSBGFP&#039;&#039;&#039;&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2302&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 2286 using <br /> <br /> 2302-SbGFP-Nhe1-F tacacgct agc gccaccATGGTGAGCAAGGGCGAG&lt;br&gt;2302-SbGFP-BamH1-R CGGTGGATCCTTCTGGAAGAAGAAATTATAAACTCCGCTTCCCTTGTACAGCT&lt;br&gt;(AGCTGTACAAGGGAAGCGGA GTT TAT AAT TTC TTC TTC CAG AAG GAT CCA CCG)&lt;br&gt;&lt;br&gt; <br /> <br /> [[Image:2273-2 2301-2 2302pcr.JPG]]&lt;br&gt; <br /> <br /> digest with BamH1 + Nhe1 <br /> <br /> [[Image:Digest for 2197 2273 2301 2302 2312.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use BamH1 + Nhe1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2197 2302 2312 2301diget.JPG]] <br /> <br /> primer design was wrong, there is no BamHI site in 2218&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 2302 pSBGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2302 pSBGFP&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2302&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 2286 using <br /> <br /> 2302-SbGFP-Kpn1-F tacacGGTACC gccaccATGGTGAGCAAGGGCGAG <br /> <br /> 2302-SbGFP-Nhe1-R GGTAGCGCTAGCTTCTGGAAGAAGAAATTATAAACTCCGCTTCCCTTGTACAGCT <br /> <br /> [[Image:2166 2302 2267PCR.JPG]]&lt;br&gt; digest with Kpn1 + Nhe1 &lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2166 2302 2267 2336PCR.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; Kpn1 + Nhe1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2302DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; Transfection <br /> <br /> [[Image:2302 pSBGFP 2014 9 14.JPG]]&lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu