https://eln.excellgen.com/index.php?action=history&feed=atom&title=2312_pEB-iFLP 2312 pEB-iFLP - Revision history 2026-10-18T16:07:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2312_pEB-iFLP&diff=12891&oldid=prev Excellgen: Created page with "== '''Cloning of 2312 pEB-iFLP''' == '''Purpose:''' Cloning of2312 pEB-iFLP'''==''' '''Date: '''2014-8---- '''Notebook:0''' '''Page:''' '''Clone ID:2312''' '''Experim..." 2014-08-22T16:51:33Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2312 pEB-iFLP&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2312 pEB-iFLP&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2312&#039;&#039;&#039; &#039;&#039;&#039;Experim...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2312 pEB-iFLP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2312 pEB-iFLP&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-8---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2312&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone from 2115 pT7A3-iFLP to 2049 pEB-N6H using nco1 + Xho1<br /> <br /> [[Image:Digest for 2197 2273 2301 2302 2312.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use HindIII to find positive clone&lt;br&gt; <br /> <br /> [[Image:2197 2302 2312 2301diget.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen