https://eln.excellgen.com/index.php?action=history&feed=atom&title=2320_pFastBacDual-VSVG-Flp 2320 pFastBacDual-VSVG-Flp - Revision history 2026-10-18T20:19:21Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2320_pFastBacDual-VSVG-Flp&diff=13250&oldid=prev Excellgen at 17:37, 24 September 2014 2014-09-24T17:37:52Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2320 pFastBacDual-VSVG-Flp&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2320 pFastBacDual-VSVG-Flp&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2320&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone Flpo from 1981 pCMV5-Tat-Flp-PDGFR to 2224 pFastbacDual-p10-VSVG-M using Nhe1 + Xba1 <br /> <br /> [[Image:Digest for 2320 2255.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; Nhe1 + Xba1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2255 2316 2320.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 2320 pFastBacDual-VSVG-Flp&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2320 pFastBacDual-VSVG-Flp&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2320&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone Flpo from 1981 pCMV5-Tat-Flp-PDGFR to 2219 pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8using Nhe1 + Xba1 <br /> <br /> [[Image:DIGEST FOR 2320PCR.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; BamHI + Xba1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2320DIGEST.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 2320 pFastBacDual-VSVG-Flp&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2320 pFastBacDual-VSVG-Flp&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2320&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone Flpo from 1981 pCMV5-Tat-Flp-PDGFR to 2219 pFastbacDual-p10-VSVG-M-6H-Tat-Flp-MFGE8usingBamH1 + Xba1 <br /> <br /> [[Image:Digest_for_2320_1038.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; BamHI + Xba1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2320_2345DIGEST.JPG]][[Image:2219_2320_1981DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen