https://eln.excellgen.com/index.php?action=history&feed=atom&title=2323_pR-Enterokinase 2323 pR-Enterokinase - Revision history 2026-10-18T16:58:48Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2323_pR-Enterokinase&diff=13446&oldid=prev Excellgen: Created page with "== '''Cloning of 2323 pR-Enterokinase-''' == '''Purpose:''' Cloning of2323 pR-Enterokinase-'''==''' '''Date: '''2014-9---- '''Notebook:0''' '''Page:''' '''Clone ID:2323..." 2014-10-20T17:11:40Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2323 pR-Enterokinase-&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2323 pR-Enterokinase-&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2323...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2323 pR-Enterokinase-&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2323 pR-Enterokinase-&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2323&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 1731 pT7A3-TurboEnterokinase <br /> <br /> Frag 1. 2323-EK-Nde1-F C1 tagca catatg attgtcggaggaagtgactc+ 2323-EK-R1 C3 tgatgccatGtgcaggcctag <br /> <br /> Frag 2 2323-EK-F1 C2 gcctgcaCatggcatcaaatc +Enteokinase-Xho1-R gtatcgCTCGAGCTAgTGATGATGATGATGATGTAGAAAACTTTGTATCCACTCTG <br /> <br /> [[Image:736fix_1916fix2_2323pcr.JPG]]&lt;br&gt; join two fragments by PCR 2323-EK-Nde1-F C1 tagca catatg attgtcggaggaagtgactc Enteokinase-Xho1-R gtatcgCTCGAGCTAgTGATGATGATGATGATGTAGAAAACTTTGTATCCACTCT <br /> <br /> [[Image:736fix_1916fix2_2323pcr2.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> digest with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:Digest_for_2362_736fix_1916fix2_2323.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; Nde1 + Xho1to find positive clone&lt;br&gt; <br /> <br /> [[Image:2323digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen