https://eln.excellgen.com/index.php?action=history&feed=atom&title=2324_pL6H-sso7d-wt-Taq 2324 pL6H-sso7d-wt-Taq - Revision history 2026-10-18T23:06:09Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2324_pL6H-sso7d-wt-Taq&diff=13062&oldid=prev Excellgen at 16:58, 11 September 2014 2014-09-11T16:58:02Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2324 pL6H-sso7d-wt-Taq&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2324 pL6H-sso7d-wt-Taq&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2324&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone from 2040 pT7A3-6H-sso7d-wt-Taq using Nco1 + Not1 to 1997 pL6H <br /> <br /> [[Image:DIGEST FOR 2315 2166 2256 2320 2332 2324.JPG]]&lt;br&gt;&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_9&lt;br&gt; <br /> <br /> Make miniprep, use Nco1 + Not1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2324DIGEST.JPG]] <br /> <br /> digest with Hindiii to verify <br /> <br /> [[Image:2135 2332 2324digest.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen