https://eln.excellgen.com/index.php?action=history&feed=atom&title=2326_pET30b-Ecoli-MutH 2026-10-18T22:23:49Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2326_pET30b-Ecoli-MutH&diff=13815&oldid=prev 2015-01-08T18:06:32Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2326 pET30b-Ecoli-MutH&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2326 pET30b-Ecoli-MutH&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2015--1- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2326&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from Escherichia coli str. K-12 substr. MG1655 genomic DNA <br /> <br /> 2326-Ecoli-MutH-Nde1-F tacgacatatgtcccaacctcgcccactg <br /> <br /> 2326-Ecoli-MutH-Xho1-R tgctactcgagctggatcagaaaatgacgggcca <br /> <br /> [[Image:2326_2327_2328pcr.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; Digest with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:Digest_for_2326_2327_2328.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2015_1&lt;br&gt; <br /> <br /> Make miniprep, use Nde1 + Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2325_2326_2327_2328digest.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

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