https://eln.excellgen.com/index.php?action=history&feed=atom&title=2328_pL42-Tth-PolyA-Pol 2328 pL42-Tth-PolyA-Pol - Revision history 2026-10-18T21:32:16Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2328_pL42-Tth-PolyA-Pol&diff=13817&oldid=prev Excellgen at 18:07, 8 January 2015 2015-01-08T18:07:37Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2328 pL42-Tth-PolyA-Pol&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2328 pL42-Tth-PolyA-Pol&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2015-1--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2328&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from Tth HB27 genomic DNA <br /> <br /> &lt;br&gt; 1 <br /> <br /> 2328-Tth-PolyA-Pol-Nde1-F atcagcatatggcccaCatggacttcccct <br /> <br /> 2328-Tth-PolyA-Pol-R ctgggcctcCaggagggcct <br /> <br /> &lt;br&gt; 2 <br /> <br /> 2328-Tth-PolyA-Pol-F aggccctcctGgaggcccag <br /> <br /> 2328-Tth-PolyA-Pol-Xho1-R TGGTGctcgagatgggggtgatctggggttc <br /> <br /> [[Image:2326 2327 2328pcr.JPG]]&lt;br&gt; <br /> <br /> 1+ 2 <br /> <br /> 2328-Tth-PolyA-Pol-Nde1-F atcagcatatggcccaCatggacttcccct <br /> <br /> 2328-Tth-PolyA-Pol-Xho1-R TGGTGctcgagatgggggtgatctggggttc &lt;br&gt; <br /> <br /> [[Image:2328PCR.JPG]]&lt;br&gt; Digest with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:Digest for 2326 2327 2328.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_9&lt;br&gt; <br /> <br /> Make miniprep, use Nde1 + Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2325 2326 2327 2328digest.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen