https://eln.excellgen.com/index.php?action=history&feed=atom&title=2349_pT7A3-Cas9-Nickase 2349 pT7A3-Cas9-Nickase - Revision history 2026-10-18T22:29:58Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2349_pT7A3-Cas9-Nickase&diff=13374&oldid=prev Excellgen at 20:19, 10 October 2014 2014-10-10T20:19:21Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2349 pT7A3-Cas9-Nickase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2349 pT7A3-Cas9-Nickase&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2349&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> hCas9-D10A-Nco1-F ctagcccatggacaagaagtactccattgggctcgCtatcggcac <br /> <br /> 2334-Cas9-BamH1-R ATGGCGGGGGATCCGGCCAGATTG &lt;br&gt; [[Image:2334 2335 2349pcr.JPG]] <br /> <br /> &lt;br&gt; digest with NCO1 + BamH1, clone to 2332 pT7A30-Cas9&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2349 2334.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp; Nco1 + BamH1 to find positive clone&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2349DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen