https://eln.excellgen.com/index.php?action=history&feed=atom&title=2356_pFastbac-GP64-iCre-iCre 2026-10-18T19:43:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2356_pFastbac-GP64-iCre-iCre&diff=13634&oldid=prev 2014-11-20T22:16:47Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2356 pFastbac-GP64-iCre-iCre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2356 pFastbac-GP64-iCre-iCre&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2356&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone from 1972 pCMV5-Tat-Cre-PDGFR to 2352 pFastbac-GP64-Cre-Flpe-WPRE using Nhe1 + Kpn1 <br /> <br /> [[Image:Digest for 2356.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use Xho1 + Kpn1 to find positive clone,will get two bands&lt;br&gt; <br /> <br /> [[Image:2356 2381 2383 2386digest&#039;.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> digest 2356-1(11/17)with NheI to comfirm,will get one band&lt;br&gt; <br /> <br /> [[Image:2356 2386digest&#039;.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

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