https://eln.excellgen.com/index.php?action=history&feed=atom&title=2369_pT7A3-Ic-MBP-%28TEV%29 2369 pT7A3-Ic-MBP-(TEV) - Revision history 2026-10-18T16:41:35Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2369_pT7A3-Ic-MBP-(TEV)&diff=13527&oldid=prev Excellgen at 21:09, 31 October 2014 2014-10-31T21:09:46Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2369 pT7A3-Ic-MBP-(TEV)&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2369 pT7A3-Ic-MBP-(TEV)&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2369&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone MBP from 2094 pT7A3-MBP-(TEV) (old name pT7A3-8H-HC-MBP-(TEV)) using Nco1 + Xho1 <br /> <br /> [[Image:Digest for2354 2369 736fix2 1916fix3 2240.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_8&lt;br&gt; <br /> <br /> Make miniprep, use BglII to find positive clone&lt;br&gt; <br /> <br /> &lt;br&gt; [[Image:736FIX2 1916FIX3 2240 2354 2369 2375.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen