https://eln.excellgen.com/index.php?action=history&feed=atom&title=237-PAd5.CMV-Cre-IRES-EGFP
237-PAd5.CMV-Cre-IRES-EGFP - Revision history
2026-10-18T08:48:42Z
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https://eln.excellgen.com/index.php?title=237-PAd5.CMV-Cre-IRES-EGFP&diff=2715&oldid=prev
Paul.xu at 20:21, 8 December 2011
2011-12-08T20:21:12Z
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<p><b>New page</b></p><div>Cloning of Cre by subclone 241 to 242<br> <br />
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Purpose: to clone 237-pAd5-Cre-GFP<br> <br />
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Date: 2011-10-14 <br />
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Notebook: luyingying <br />
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Page: <br />
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Clone ID:237 <br />
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Experimenters: lu yingying <br />
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Result <br> <br />
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[[Image:237subclone10-13-2011.JPG]] <br />
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band shows vector <br />
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[[Image:237vector10-14-2011.JPG]] <br />
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bands show cre<br> <br />
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Restart<br> <br />
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Date: 2011-10-18 ---<br> <br />
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Notebook: luyingying <br />
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Page: <br />
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Clone ID:237 <br />
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Experimenters: lu yingying <br />
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Result <br> <br />
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[[Image:237 crePCR product10-18-2011.JPG]]<br> <br />
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PCR product <br> <br />
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298 plasmid as template<br> <br />
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primer:Lenti-Cre-F+Lenti-Cre-R<br> <br />
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25 cycles <br />
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[[Image:237 vector+cre digest 10-19-2011.JPG]]<br> <br />
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digest resulte:EcoR1+ Xho1 NEB enzyme 37 for 3 hours<br> <br />
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242 plasmid&nbsp; digest as vector<br> <br />
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<br> <br />
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Cloning of Cre by subclone 298 pTriEx-HTNC (Cre) to 242 pAd5-CMV-2-IRES-GFP<br> <br />
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Purpose: to clone 237-pAd5-Cre-GFP <br />
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Date: 2011-10-25 <br />
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Notebook: luyingying <br />
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Page: <br />
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Clone ID:237 <br />
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Experimenters: lu yingying <br />
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Result <br> <br />
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[[Image:470+359+237cloning 10-25-2011.JPG]]<br> <br />
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band 4,5 show subclone result:EcoR1+ Xho1<br> <br />
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band 4 show 242 pAd5-CMV-2-IRES-GFP digest result<br> <br />
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band 5 show 298 pTriEx-HTNC (Cre) digest result<br> <br />
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[[Image:237 digest detect10-27-2011.JPG]]<br> <br />
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band 1-6 show digest detect result:EcoR1+ Xho1<br> <br />
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Date: 2011-11-10 --- <br />
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Notebook: luyingying <br />
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Page: <br />
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Clone ID:237 <br />
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Experimenters: lu yingying <br />
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Result <br> <br />
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[[Image:237 crePCR product10-18-2011.JPG]] <br />
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PCR amplify His-Tat-NLS-Cre(298) as temlate, using Cre-EcoR1-F B1&nbsp; + Cre-BglII-R B2<br> <br />
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[[Image:237+469 digest11-9-2011.JPG]] <br />
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PCR product with EcoR1 + BglII, digest pAd5-CMV-IRES-GFP with BamH1 + EcoR1<br> <br />
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band 1 show insert, band 2 show pAd5-CMV-IRES-GFP(242) vector digest<br> <br />
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[[Image:237+469+493 digest detect 11-14-2011.JPG]] <br />
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&nbsp;digest detect:EcoR1+ Not1<br> <br />
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Date: 2011-11-16 --- <br />
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Notebook: luyingying <br />
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Page: <br />
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Clone ID:237 <br />
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Experimenters: lu yingying <br />
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Result <br> <br />
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[[Image:237-PCR 11-16-2011.JPG]]<br> <br />
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PCR amplify His-Tat-NLS-Cre(298) as temlate, using Cre-EcoR1-F2 B1 + Cre-BglII-R B2<br> <br />
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[[Image:237 insert+vector11-17-2011.JPG]]<br> <br />
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PCR product with EcoR1 + BglII, digest pAd5-CMV-IRES-GFP with BamH1 + EcoR1<br> <br />
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[[Image:237 digest detect11-21-2011.JPG]] <br />
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digest test:EcoR1+Not1 <br />
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[[Category:Cloning]]</div>
Paul.xu