https://eln.excellgen.com/index.php?action=history&feed=atom&title=2373_pEC2-Trp63 2026-10-19T03:33:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2373_pEC2-Trp63&diff=13549&oldid=prev 2014-11-03T17:36:32Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2373 pEC2-Trp63&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2373 pEC2-Trp63&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2373&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> Gene synthesis fragment 1 &lt;br&gt; <br /> <br /> PCR from 2372 pSpeedET-Trp63 (fragment 2)&lt;br&gt; <br /> <br /> Gene Synthesis Fragment 3 <br /> <br /> [[Image:2373pcr1.JPG]]&lt;br&gt; <br /> <br /> join 3 fragments by pcr&lt;br&gt; <br /> <br /> [[Image:2371_2373PCR.JPG]]&lt;br&gt; <br /> <br /> digest with BamH1 + HindIII, clone to pEC1454&lt;br&gt; <br /> <br /> [[Image:DIGEST_FOR_2371_2373.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_9&lt;br&gt; <br /> <br /> Make miniprep, use BamH1 + HindIII to find positive clone&lt;br&gt; <br /> <br /> [[Image:2373DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

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